The updated manual now includes a mandatory replacement of the rubber motor mounts if the centrifuge is used for qPCR prep (low vibration requirement). The old rubber compound hardens and transmits vibration after 8 years.
: The 5402 features adjustable speeds from 1,000 to 14,000 RPM (approx. 16,000 x g).
Opposing tubes must contain fluid of equal density and equal volume (within a tolerance of 0.1 grams).
Remove the rotor fully from the spindle motor. Clean the tube buckets and the underside of the rotor. eppendorf 5402 manual updated
If you are using the original 1990s or early 2000s instruction booklet, you are likely missing critical updates. Since the centrifuge’s production ceased, Eppendorf has released several (TI sheets) and safety updates that supersede the original manual.
If you have a specific question not covered here or need to share other tips from your experience, ask away, and I'll do my best to help!
This article serves as a comprehensive resource, compiling the most critical information from the centrifuge's original documentation and user community knowledge. The updated manual now includes a mandatory replacement
In case of tube breakage or biological spills, disinfect the rotor and chamber immediately using a 70% isopropyl alcohol solution or a neutral laboratory disinfectant. Avoid harsh bleach solutions that corrode the aluminum rotor.
Understanding the fundamental capabilities of the Eppendorf 5402 ensures you operate the unit within its intended limits, protecting both the operator and the samples. Key Specifications 14,000 RPM (Revolutions Per Minute).
Never attempt this while the rotor is still spinning. Always look through the viewing window in the lid to ensure complete standstill. 7. Decontamination and Disposal If hazardous material spills inside the chamber: Turn off the machine immediately. 16,000 x g)
The Eppendorf Centrifuge 5402 is a classic, high-performance refrigerated microcentrifuge designed for quick separation of liquid substance mixtures. Known for its robust build and reliability, this laboratory workhorse remains a staple in molecular biology, biochemistry, and clinical research facilities worldwide.
Search the official Eppendorf Technical Support forum using the tag #Eppendorf5402Update or contact their legacy equipment team directly – they still support this legendary centrifuge.
| Parameter | Value | |-----------|-------| | Max. capacity | 24 × 2.0 mL | | Max. speed | 14,000 rpm | | Max. RCF | 16,000 × g | | Time range | 0–99 min / continuous | | Noise level | < 60 dB(A) | | Dimensions (W×D×H) | 28 × 35 × 24 cm | | Weight | ~10 kg | | Power supply | 230V / 50-60 Hz or 115V (model dependent) |
Replace the rubber chamber gasket if it becomes cracked or brittle to maintain accurate temperature insulation.
Press the button. The centrifuge will smoothly accelerate to the target RPM.